A recently purchased state-of-the-art JEOL JEM 3200FS transmission electron microscope (TEM) is shared between the Departments of Biochemistry, Chemistry, and Biology. This instrument is designed for low dose, low temperature imaging and tomography of biological materials. The electron microscope is also equipped with an energy filter for zero-energy-loss imaging (for biological samples), elemental imaging and electron energy loss spectroscopy (EELS), a high angle annular dark field scanning TEM (HAADF-STEM) detector for Z-contrast imaging and an energy dispersive X-ray (EDX) spectrometer. Though these add-ons are mainly used for materials science applications, recent work has begun to explore the use of such materials science instrumentation with biological materials and the electron microscope here at IU is ideally suited for such explorations. The facility is also equipped with an FEI Vitrobot for robotic vitrification of isolated macromolecular assemblies. The manager of the TEM will assist with initial feasibility studies and project design, data processing and analysis and training users of the instrument.
Location: SI 032